PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF DIFFERENT SOLVENT EXTRACTS FROM Annona squamosa Linn. LEAVES
M. L. MOHAMMED KALEEM ARSHAN *
Department of Biotechnology, Islamiah College (Autonomous), Vaniyambadi, India.
FARHANAAZ MAGI
Department of Botany, Secab ARS Inamdar Arts, Science and Commerce Degree College for Women, Vijayapur, India.
REENU MISHRA
Department of Botany, A.P.S. University, Rewa, India.
*Author to whom correspondence should be addressed.
Abstract
Plants are the chief source of natural compounds used for medicine, in which medicinal plants have attracted considerable interest and most attention for their wide variety of bioactive metabolites. Numerous studies have been carried out to screen extracts from medicinal plants for the presence of novel compounds and an investigation of their biological activities. Annona squamosa Linn. has extensively been used in the traditional and folkloric medicine and found to possess many biological activities. This study was carried out to evaluate the phytochemical screening and antioxidant activities of acetone, chloroform, hexane, methanol, Petroleum ether and aqueous extracts of Annona squamosa Linn. leaves. The antioxidant properties were determined by scavenging 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH) method. Results of phytochemical analysis of extracts showed the presence of glycosides, saponins, tannins, flavonoids, phenols, etc. In-vitro antioxidant activities clearly suggest that methanol extract has higher antioxidant activity than the other extract due to a higher presence of phenolic and flavonodal constituents in the methanol extract. These experimental findings would further establish the scientific basis of the traditional uses of the plant in the management of different conditions as well as control of different disease.
Keywords: Bombyx mon Directional selection, Annona squamosa, pupal weight, antioxidant activity, cocoon shell percentage and fecundity, phytochemical screening, free radicals.
How to Cite
Downloads
References
Velioglu YS, Mazza G, Gao L, Oomah D. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. J Agric Food Chem. 1998;46:4113-4117.
Vasundhara S, Kumar HV, Rao JM. Influence of milk and sugar on antioxidant potential of black tea. Food Res Int. 2008;41:124-129.
Li HY, Hao ZB, Wang XL, Huang L, Li JP. Antioxidant activities of extracts and fractions from Lysimachia foenum-graecum Hance. Bioresour Technol. 2009;100:970-974.
Meghashri S, Vijay Kumar H, Gopal S. Antioxidant properties of a novel flavonoid from leaves of Leucas aspera. Food Chem. 2010;122:105-110.
Roy N, Laskar RA, Sk I, Kumari D, Ghosh T, Begum NA. A detailed study on the antioxidant activity of the stem bark of Dalbergia sissoo Roxb. an Indian medicinal plant. Food Chem. 2011;126:1115-1121.
Formica JV, Regelson W. Review of the biology of quercetin and related bioflavonoids. Food Chem Toxicol. 1995;33:1061-1180.
Yoganarasimhan SN. Medicinal Plants of India, Tamil Nadu. Bangalore: Cyber Media. 2000;248.
Nadkarni AK. Indian Materia Medica. Mumbai: Popular Prakashan Ltd. 2000;I:116.
Roopana SM, Bharathi A, Kumar R, Khanna VG, Prabhakarn A. Acaricidal, insecticidal, and larvicidal efficacy of aqueous extract of Annona squamosa L peel as biomaterial for the reduction of palladium salts into nanoparticles. Colloids Surf B Biointerfaces. 2012;92:209-221.
Kumar R, Roopan SM, Prabhakarn A, Khanna VG, Chakroborty S. Agricultural waste Annona squamosal peel extract: Biosynthesis of silver nanoparticles. Spectrochim Acta A Mol Biomol Spec-Trosc. 2012;90:173-176.
Celeghini RMS, Vilegas JHY, Lanças FM. Extraction and quantitative HPLC analysis of coumarin in hydroalcoholic extracts of Mikania glomerata Spreng. (“guaco”) leaves. J Braz Chem Soc. 2001;12:706-709.
Sofowora A. Phytochemical screening of medicinal plants and traditional medicine in Africa, Spectrum Books Ltd., Ibadan, Nigeria; 1993.
Trease GE, Evans WC. Phenols and phenolic glycosides, in textbook of pharmacognosy. Balliese, Tindall and Co Publishers, London, UK. 1989;12:343–383.
Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Food Sci Technol. 1995; 28:25–30.
Lapin Lawrence L. Modern engineering statistics. Belmont; London. 1997;583.
David E, Elumalai EK, Sivakumar C, Therasa SV, Thirumalai T. Evaluation of antifungal activity and phytochemical screening of Solanum surattense seeds. J Pharm Res. 2010; 3:684-687.
Lacaille-Dubois MA, Wagner H. A review of the biological and pharmacological activities of saponins. Phytomedicine. 1996;2:363-386.
Omulokoli E, Khan B, Chhabra S. Antiplasmodial activity of four Kenyan medicinal plants. J Ethnopharmacol. 1997;56: 133-137.
Mahato SB, Sen S. Advances in triterpenoid research, 1990–1994. Phytochemistry. 1997; 44(7):1185–1236.
Yermilov V, Rubio J, Becchi M, Friesen MD, Pignatelli B, Ohshima H. Formation of 8-nitroguanine by the reaction of guanine with peroxynitrite in vitro. Carcinogenesis. 1995;16: 2045-2050.
Dehpour AA, Ebrahimzadeh MA, Nabavi SF, Nabavi SM. Antioxidant activity of methanol extract of Ferula asafoetida and its essential oil composition. Grasas Aceites. 2009;60: 405-412.
Chou ST, Chao WW, Chung YC. Antioxidative activity and safety of 50% ethanolic red bean extract (Phaseolus radiatus L. var. aurea). J Food Sci. 2003;68:21-25.
Shahidi F, Wanasundara PK. Phenolic antioxidants. Crit Rev Food Sci Nutr. 1992;32: 67-103.
Kalidindi N, Thimmaiah NV, Jagadeesh NV, Nandeep R, Swetha S, Kalidindi B. Antifungal and antioxidant activities of organic and aqueous extracts of Annona squamosa Linn. leaves. Journal of Food and Drug Analysis, 2015;23(4):795–802.
Biva IJ, Islam MM, Saha MR, Islam MS, Sarwar S. In vitro antioxidant and cytotoxicity studies of Annona squamosa Linn. Stamford J. Pharmaceut. Sci. 2009;2(1):32-36.
Shirwaikar A, Rajendran K, Dinesh Kumar C, Ram Gopal B. In vitro antioxidant studies of Annona squamosa Linn. leaves. Ind. J. Exp Biol. 2017;45(9):123-126.
Do QD, Angkawijaya, AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S, Ju Y. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatic. J Food Drug Anal. 2014;22:296-302.