BIOLOGICAL AND GENETIC OVERVIEW OF Pectinophora gossypiella AND ITS ASSOCIATED PARASITOID Bracon brevicornis GENOMES USING TWO MOLECULAR MARKERS TECHNIQUES
MERVAT A. KANDIL
Plant Protection Research Institute, Agriculture Research Center, Dokki - Giza, Egypt.
HEMAT Z. MOUSTAFA *
Plant Protection Research Institute, Agriculture Research Center, Dokki - Giza, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Bachground: The comparative between the long-term autumn storage Pectinophora gossypiella diapauses larvae (field strain) (durations extended to 4 months) with active larvae (laboratory strain) and its associated field parasitoid Bracon brevicornis were investigated.
Results: The mean duration of the total immature stages of B. brevicornis was 12.5 and 10.0 days, respectively, when reared on P. gossypiella diapaused larvae (field strain) and active laboratory strain. The generation time of B. brevicornis was 19.2 when reared on field diapaused larvae and 14.9 days for laboratory strain. The Sex ration of B. brevicornis estimated by (0.6:0.4) in active larvae and (0.55:0.45) in diapause larvae. The percentage of produced females in progeny was significantly different from that of two cultured parasitoids. Also, results revealed that adult male numbers decreased than female which considerable necessary in the B. brevicornis progeny production of following generations. The total eggs number per B. brevicornis female was 158.0 when reared on diapaused larvae of P. gossypiella field strain, while it increased to 236.0 when reared on active larvae of P. gossypiella laboratory strain. Identification of P. gossypiella and B. brevicornis genomes using two different techniques; SCoT method which conjugate with genes for the first time in Egypt on the host and its parasitoid and ISSR method which conjugate with sequences.
Conclusion: using the two techniques had been recognized the most parts of P. gossypiella and B. brevicornis genomes, our findings may be helpful in genetic and genomic studies.
Keywords: Pectinophora gossypiella, diapauses, active larvae, Bracon brevicornis, biology, genetic profile, SCoT, ISSR
How to Cite
Downloads
References
HagleyEAC, Barber DR. Effect of food sources on the longevity and fecundity (of Pholetesor ornigis Weed) (Hym.: Braconidae). Can. Ent. 1992;124:341–346.
RidgwayNM, Mahr DL. Reproduction, de development, and longevity of Pholetesor ornigis (Hymeonoptera: Braconidae), a parasitoid of spottedtenti form leaf miner (Lepidoptera; racillaridae), in the laboratory. Ann. Entomol. Soc. Am. 1990;83:790-794.
Kandil MA, Moustafa HZ. Impact of conventional insecticides on two bollworms and their indirect effects on the ectoparasitoid, Bracon brevicornis (Wesmael (Hymenoptera: (Braconidae(.Bulletin of the National Research Centre. 2019;43:202
Available: https://doi.org/10.1186/s42269-019-0250-3.
Kandil MA, Moustafa HZ, Lotfy DE. Behavior and generation times of Bracon brevicornis (Wesmael) )Hymenoptera:Bracondae) reared on Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) and Galleria mellonella (Lepidoptera: Pyralidae). International Journal of Tropical Insect Science; 2020.
Available:https://doi.org/10.1007/s42690-020-00312-7.
Noble LW. The biological possibility of infestation by flight of the pink bollworm moth. Journal of Economic Entomology. 1936;29:78–79.
Phillips TW. The science and technology of post harvest insect control challenges, accomplishments and future directions. In: Heaps, J.W. (Ed.), Insect Management for Food Storage and Processing, second ed. American Association of Cereal Chemist. 2006;211-222.
Phillips TW, Throne JE. Biorational approaches to managing stored-product insects. Annual Review of Entomology. 2010;55:375-397.
Collard BCY, Mackill DJ. Start Codon Targeted (SCOT) Polymorphism: a simple novel DNA marker technique for generating gene-targeted markers in plants. Plant Mol. Biol. Rep. 2009;27:86–93. Available:https://doi.org/10.1007/s11105-008-0060-5.
Reddy M, Sarla PN, Siddiq EA. Inter simple sequence re-peat (ISSR) polymorphism and its application in plant breeding. Euphytica. 2002;128:9-17.
Havlíčková L, Jozová E, Rychlá A, Klima M, Kučera V, Čurn V. Genetic diversity assessment in winter oilseed rape (Bras-sicanapus L.) collection using AFLP, ISSR and SSR markers. Czech Journal of Genetics and Plant Breeding. 2014;50:216-225.
Levy Hazel C, Garcia-Maruniak Alejandra, Maruniak James E. Strain identification of Spodoptera frugiperda (Lepidoptera: Noctuidae) insects and cell line: PCR-RFLP of cytochrome oxidase C subunit I gene. Florida Entomologist. 1992;85(1):186-190.
Available:https://doi.org/10.1653/0015-4040.
Amer AEA. Economic artificial diets for rearing spiny bollworm, Earias insulana (Boisd.) (Lepidoptera: Noctuidae). J Plant Prot Pathol, Mansoura Univ. 2015;6 (3):527 – 534.
Williams, JGK, Kubelk AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acid Res. 1990;18:6231-6235.
Yang X, Quiros CF. Identification and classification of celery cultivars with RAPD markers. Theoritical and Appleid Genetics. 1993;86:205-212.
Farag, RS, Omar AMA. Fatty Acid Composition of Lipids Extracted from Pink Bollworm in Relation to Dietary Lipids — Induction and Termination of Diapause. European Journal of lipid science and technology. 1981;83:271-274.
Thanavendan G, Jeyarani S. Effect of different temperature regimes on the biology of Bracon brevicornis Wesmael (Braconidae: Hymenoptera) on different host larvae. Journal of Bio-pesticide. 2010;3(2): 441-444.
Lee JC, Heimpel GE. Effect of floral nectar, water, and feeding frequency on Cotesia glomerata longevity. Biol. Control. 2008;53:289–294.
Available:https://doi.org/10.1007/s10526-007-9070-8
Winkler K, Wäckers FL, Kaufman LV, Larraz, V, Lenteren JCV. Nectar exploitation by herbivores and their parasitoids is a function of flower species and relative humidity. Biol. Control. 2009;50:299–306
Available: https://doi.org/10.1016/j.biocontrol.2009.04.009
Chen HH, Zhang, Zhu KY, Throne J. Performance of diapausing parasitoid wasps Habrobracon hebetor, after cold storage. Biol. Control. 2013;64(3):186-194.
Frere I, Balthazar C, Sabri A, Hance T. Improvement in the cold storage of Aphidius ervi (Hymenoptera: Aphidiinae). Eur. J. Environ. Sci. 2011;1(1):33-40.
Chen, H, Opit GP, Sheng P, Zhan H. Maternal and progeny quality of Habrobracon hebetor (Say) (Hymenoptera: Braconidae) after cold storage. Biol.Contrl. 2011;58:255–261.
Kandil MA. Studies on the predaceous and parasitic insects on the pink and spiny bollworm. Ph.D. Thesis, Faculty of Agriculture, Benha Branch, Zagazig University; 2001.
Colinet H, Boivin G. Insect parasitoids in cold storage: A comprehensive review of factors of variability and consequences. Biol. Contrl. 2011;58:83–95.
Endersby NM, Hoffmann AA, McKechnie SW, Weeks AR. Is there genetic structure in populations of Helicoverpa armigera from Australia? Entomol Exp Appl. 2007;122:253–263.
Luque C, Legal L, Staudter H, Gers C, Wink M. ISSR (Inter Simple Sequence Repeats) as genetic markers in Noctuids (Lepidoptera). Hereditas. 2002;136:251–253.
Fang Chen, Juan Shi, You-qing Luo, Shuang-yan Sun, Min Pu. Genetic Characterization of the Gypsy Moth from China (Lepidoptera, Lymantriidae) Using Inter Simple Sequence Repeats Markers; 2013.
Moustafa Hemat Z, Kandil MA, El-Bassouiny HM. Genetic profile characterization of Earias insulana (Lepidoptera:Noctuidae) in Egypt Egypt. J. Plant Prot. Res. Inst. 2021;4(2):341–351.